Western blotting, also known as immunoblotting, is one of the most widely-used foundational techniques for the identification and quantitation of protein expression. Furthermore, this technique can be used to understand how proteins interact with DNA, to measure protein modification such as phosphorylation or glycosylation and to evaluate different protein isoforms. Advances have been made in all aspects of the technique, from sample preparation to labelling and visualization of proteins, as well as automation.
In this article collection, we present a series of review and research articles that illustrate the power of western blotting and recent advances in the underlying techniques as well as new approaches. Our goal is to educate the reader on both fundamental and new techniques in sample preparation, automated processing, and more to elevate the quality and depth of western blot results.
What you will learn:
- Fundamentals of the western blot technique
- Different methods for detecting proteins of interest
- Applications for western blotting in both the research and clinical setting
Articles contained in the collection:
Meftahi, GH, Bahari, Z, Zarei Mahmoudabadi, A, Iman, M, Jangravi, Z. Applications of western blot technique: From bench to bedside. Biochem Mol Biol Educ. 2021; 49: 509– 517.
Ni, D., Xu, P., and Gallagher, S. 2017. Immunoblotting and immunodetection. Curr. Protoc. Protein Sci. 88: 10.10.1- 10.10.37.
Gong, J.-H., Gong, J.-P. and Zheng, K.-W. (2017), Checking transfer efficiency and equal loading via qualitative optical way in western blotting. ELECTROPHORESIS, 38: 2786-2790.
Ni, D., Xu, P., Sabanayagam, D. and Gallagher, S.R. 2016. Protein blotting: immunoblotting. Curr. Protoc. Essential Lab. Tech. 12: 8.3.1- 8.3.40.
Berkelman, T. (2020). Fluorescent western blotting: High sensitivity detection of multiple targets. Current Protocols in Pharmacology, 88, e72.