Advances in Bioprocessing Cell Line Development
The advent of engineered recombinant proteins for use as therapeutic agents, termed biologics, has led to an explosion in the number of treatments available for a range of diseases. The process of creating biologic-producing cells is called cell line development. It encompasses the engineering of a host cell line to express a designed therapeutic molecule for the purpose of commercial production, which can take months to years to accomplish. For any cell line development pipeline, there are key consideration such as accurate measurements of cell line growth and productivity, biologic product quality, and costs associated with reagents and labor required to maintain the cultures leading up to commercial production.
In this article collection, we present recent advances in cell line development for biological-based therapeutics. These new technologies and methodologies are accelerating the release of therapeutics to market by enabling pharmaceutical companies to generate, screen and characterize large numbers of cell lines in parallel much faster, and with more accuracy and fidelity than ever before.
What you will learn:
- Methodology to improve your clonal cell production and screening
- The value of automated screening technologies during bioprocessing
- Application examples of next-generation processes to biologic production
Articles contained in the collection:
Huhn, SC, Ou, Y, Tang, X, et al. Improvement of the efficiency and quality in developing a new CHO host cell line. Biotechnol Progress. 2021; 37( 5):e3185.
Kuhn, A, Le Fourn, V, Fisch, I, Mermod, N. Genome-wide analysis of single nucleotide variants allows for robust and accurate assessment of clonal derivation in cell lines used to produce biologics. Biotechnology and Bioengineering. 2020; 117: 3628– 3638.
Markert, S., Musmann, C., Hülsmann, P. and Joeris, K. (2019), Automated and enhanced clone screening using a fully automated microtiter plate-based system for suspension cell culture. Biotechnol. Prog., 35: e2760.
Li, X, Zhang, Y, Jing, L, et al. Integration of high-throughput analytics and cell imaging enables direct early productivity and product quality assessment during Chinese Hamster ovary cell line development for a complex multi-subunit vaccine antigen. Biotechnol Progress. 2020; 36:e2914.
Kreye, S, Stahn, R, Nawrath, K, Goralczyk, V, Zoro, B, Goletz, S. A novel scale-down mimic of perfusion cell culture using sedimentation in an automated microbioreactor (SAM). Biotechnol Progress. 2019; 35:e2832.
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