Induced pluripotent stem cells (iPSCs) are rapidly becoming the gold standard system for drug development, as well as a scalable source for cell-based therapies. Characterization of cell lines from a variety of sources to ensure quality and cell fate are key steps in their production. Advanced flow cytometry methods are an instrumental part of the characterization process, able to accurately and efficiently phenotype cell parameters and fate during different stages of derivation and expansion.
In this article collection, we seek to highlight six recent publications that illustrate not only the use of pluripotent stem cells for cell-based therapies but how flow cytometry can be utilized to measure cell parameters. We hope to provide readers a deeper appreciation for the utility and role of advanced flow cytometry in the production, characterization, and expansion of induced pluripotent stem cells cultures for clinically relevant applications.
What you will learn:
- Advanced flow cytometry for iPSC characterization
- Considerations for scaling of iPSC cultures
- Emerging culturing iPSC culturing techniques.
Articles contained in this collection:
Rimac, V, Bojanić, I. (2022), Role of flow cytometry in evaluation of the cellular therapy products used in haematopoietic stem cell transplantation. International Journal of Laboratory Hematology.
Kim, M.-H., et al. (2023), Novel strategy to improve hepatocyte differentiation stability through synchronized behavior-driven mechanical memory of iPSCs. Biotechnology and Bioengineering.
Du, W., et al. (2022), Generation of universal natural killer cells from a cryopreserved cord blood mononuclear cell-derived induced pluripotent stem cell library. FEBS Open Bio.
Torizal, F. G., et al. (2022), Production of homogenous size-controlled human induced pluripotent stem cell aggregates using ring-shaped culture vessel. Journal of Tissue Engineering and Regenerative Medicine.
Cole, D., et al. (2022) Characterization and Optimization of Induced Pluripotent Stem Cell Culture Using Advanced Flow Cytometry and Live-Cell Analysis. Application Note. Sartorius.
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