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Cellular therapy using transplantation of stem cells collected from bone marrow and peripheral blood are key treatments for diseases of the hematopoietic system. Crucial to this is a careful and reliable measurement of the viability and quantity of stem cells available for transplantation and the outcomes post-transplantation. Measurement of the hematopoietic stem cells is commonly done using the International Society of Hemotherapy and Graft Engineering (ISHAGE) protocol, but variations in methodology can lead to reproducibility and standardization issue. Optimally configured flow cytometry equipment such as the BD FACSLyric flow cytometer along with reliable labelling reagents such as the BD™ Stem Cell Enumeration kit have greatly improved the evaluation of HSCs collected from bone marrow or peripheral blood collection.
In this article collection, we present best practices and methodology for clear and accurate measurements of CD34+ stem cells in the process of collecting and enumerating PBSCs for transplantation-based therapeutics. By introducing readers to the advantages of clinical flow cytometry combined with the BD Stem Cell Enumeration kit, we hope to empower users to investigate the use of this technology to address their specific diagnostic goals.
What you will learn:
- Advances in flow cytometry for stem cell enumeration.
- Diagnostic labeling of peripheral blood cell populations.
- Evaluation of different technologies for stem cell enumeration.
Articles contained in the collection:
Barcia Durán, J.G., Lis, R. and Rafii, S. (2019), Haematopoietic stem cell reprogramming and the hope for a universal blood product. FEBS Lett.
Rimac, V, Bojanić, I. (2022), Role of flow cytometry in evaluation of the cellular therapy products used in haematopoietic stem cell transplantation. Int J Lab Hematol.
Chung, Y, Kong, JH, Hu, Y, et al. (2021), Comparison of spectra optia and amicus cell separators for autologous peripheral blood stem cell collection. J Clin Apher.
Boehlen, R, Fernandez, P, Bargetzi, M, Sigle, J-P. (2020), A new protocol for improving efficiency of autologous peripheral blood stem cell collection in patients with high white blood cell counts. J Clin Apher. 2020; 35: 217– 223.
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