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Through this Article Collection, we hope to educate researchers on oligo pools and potential applications in functional genomics pooled screening and synthetic biology.
What you will learn about:
- Splicing assays in precision medicine
- Gene assembly using custom oligo pools
- Variant library construction for protein and enzyme engineering
More Information
Pooled oligo synthesis involves the massively parallel chemical synthesis of thousands of unique oligo sequences on a silicon-chip substrate. Once synthesized, the single-stranded oligos are released from the substrate and combined into a single library of custom oligo sequences. Oligo pools are cost-effective tools for generating the diverse synthetic DNA needed for high-throughput genomics screening and advanced protein and pathway engineering. Compared to traditional sources of custom synthetic DNA, pooled oligo synthesis provides more cost-effective methods of gene assembly, higher throughput, and more efficient genomics research in massively parallel reporter assays (MPRA) or pooled CRISPR screening. High-resolution DNA writing platforms offer researchers a powerful technology for custom synthetic DNA production bringing speed and scalability to almost any application.
Articles contained in the collection:
- Rhine, C.L. et al. (2019). Future directions for high-throughput splicing assays in precision medicine. Human Mutation. DOI: 10.1002/HUMU.23866.
- Kuiper, B.P. et al. (2022). Oligo Pools as an Affordable Source of Synthetic DNA for Cost-Effective Library Construction in Protein- and Metabolic Pathway Engineering. ChemBioChem. DOI: 10.1002/CBIC.202100507.
- Eroshenko, N. et al. (2012). Gene Assembly from Chip-Synthesized Oligonucleotides. Current Protocols in Chemical Biology. DOI: 10.1002/9780470559277.CH110190.
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