Extracellular vesicles (EVs) are tiny particles surrounded by membranes that cells produce and use to communicate with one another. These particles contain distinct surface markers, proteins, and nucleic acids that reveal information about the cells that secreted them. Flow cytometry is a useful tool for studying EVs because it can measure individual particles’ size, concentration, and surface markers, even in a mixed sample. Flow cytometry is utilized in evaluating EVs, using standardized configurations to ensure consistent outcomes. By identifying EV subpopulations that are associated with disease, flow cytometry can assist in detecting, diagnosing, and tracking disease progression.
In this article collection, we present a comprehensive overview of the potential of flow cytometry as a tool in EV research, diagnostics, and therapeutics. The collection emphasizes the significance of standardization in EV analysis. Our aim is to empower users to explore the potential of utilizing flow cytometry for their specific EV research objectives by highlighting its advantages.
What you will learn:
- Advances in flow cytometry for EV research and analysis.
- EV preparation for analysis by flow cytometry.
- Evaluation of different protocols for EV flow experiments.
Articles contained in the collection:
Couch, Y., Buzàs, E. I., Di Vizio, D., Gho, Y. S., Harrison, P., Hill, A. F., Lötvall, J., Raposo, G., Stahl, P. D., Théry, C., Witwer, K. W., & Carter, D. R. F. (2021). A brief history of nearly EV-erything – The rise and rise of extracellular vesicles. J. Extracell. Vesicles
Rikkert, L.G., van der Pol, E., van Leeuwen, T.G., Nieuwland, R. and Coumans, F.A.W. (2018), Centrifugation affects the purity of liquid biopsy-based tumor biomarkers. Cytometry
Welsh, J.A., Tang, V.A., van der Pol, E. and Görgens, A. (2021), MIFlowCyt-EV: The Next Chapter in the Reporting and Reliability of Single Extracellular Vesicle Flow Cytometry Experiments. Cytometry