Advances in Downstream Processing of
Complex Antibodies

Article Collection

Therapeutic antibodies have become one of the most rapidly growing classes of drugs, revolutionizing modern medicine. Optimized manufacturing and purification processes are critical to ensure the quality, safety, and efficacy of monoclonal antibody (mAb) products, as well as to ensure the widescale use of mAb therapeutics in patients.

As emerging mAbs and next-generation antibodies continue to grow in complexity, traditional capture chromatography approaches may be suboptimal or completely inadequate, creating a need for novel purification solutions. To overcome these challenges, bioprocessing scientists have been creating innovative resins in both the capture and polish steps of complex antibody purification. Together, the goal of these solutions is to simplify the downstream processing of newly-developed complex mAbs and mAb fragments, increasing the overall yield and purity of the final product.

Through this research article collection, we hope to educate downstream processing scientists on novel solutions that can be used to enhance purification of complex mAbs.

What you will learn:

  • Key considerations for selecting an appropriate affinity chromatography ligand
  • Utility of novel affinity resins in developing simplified, efficient next-generation complex antibody purification protocols
  • Simple, effective and efficient methods for complex mAb polishing


  • Spooner et al. (2015). Evaluation of strategies to control Fab light chain dimer during mammalian expression and purification: A universal one-step process for purification of correctly assembled Fab.
  • Dennler et al. (2015). Microbial Transglutaminase and c-myc-Tag: A Strong Couple for the Functionalization of Antibody-Like Protein Scaffolds from Discovery Platforms.
  • Perret et al. (2015). The quest for affinity chromatography ligands: are the molecular libraries the right source?
  • Masuda et al. (2019). Cation exchange chromatography performed in overloaded mode is effective in removing viruses during the manufacturing of monoclonal antibodies.
  • ThermoFisher Scientific (2018). Efficient removal of aggregates from monoclonal antibodies by hydrophobic interaction chromatography in flow-through mode.

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